By Michio Hiroshima, Yasushi Sako (auth.), Yasushi Sako, Masahiro Ueda (eds.)

ISBN-10: 9048198631

ISBN-13: 9789048198634

ISBN-10: 904819864X

ISBN-13: 9789048198641

This ebook encompasses the intriguing advancements and demanding situations within the fast-moving and speedily increasing learn box of single-molecule kinetic research of phone signaling that can provide to be the most major and interesting components of organic examine for the foreseeable destiny. mobile signaling is conducted via advanced response networks of macromolecules, and single-molecule analyses has already verified its strength to solve complicated response dynamics in purified platforms. to this point, many of the released examine within the box of single-molecule procedures in cells, specialise in the dynamic houses (translational pursuits of the centre of mass) of organic molecules. although, we are hoping that this ebook offers as many kinetic analyses of mobile signaling as attainable. even supposing single-molecule kinetic research of mobile structures is a comparatively younger box compared to the research of single-molecule pursuits in cells, this kind of research is extremely vital since it at once pertains to the molecular capabilities that regulate mobile habit and sooner or later, single-molecule kinetic research may be mostly directed in the direction of mobile structures. hence, we are hoping that this publication could be of curiosity to all these operating within the fields of molecular and mobilephone biology, in addition to biophysics and biochemistry.

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Defize LHK, Arndt-Jovin DJ, Jovin TM, Boonstra J, Meisenhelder J, Hunter T, de Hey HT, de Laart SW (1988) A431 cell variants lacking the blood group A antigen display increased high affinity epidermal growth factor-receptor number, protein-tyrosine kinase activity receptor turnover. J Cell Biol 107:939–949 21. Delcroix JD, Valletta JS, Wu C, Hunt SJ, Kowal AS, Mobley WC (2003) NGF signaling in sensory neurons: evidence that early endosomes carry NGF retrograde signals. Neuron 39:69–84 22. Downward J, Parker P, Waterfield MD (1984) Autophosphorylation sites on the epidermal growth factor receptor.

The TrkA dimers are then endocytosed, trafficked to signaling endosomes [21, 32], and transported retrogradely to the soma [37]. Previous studies have demonstrated NGF properties in terms of its mobility, clustering [50], interaction kinetics with its receptor [43], internalization, and transport [8, 30, 49]. Fluorescence-labeled NGF has been used in these studies to image the somata of sensory neurons, but not single molecules. 1 Single-Molecule Behavior of NGF on the Growth Cone The b subunit of NGF (bNGF) exists as a dimer of two identical 13-kDa polypeptides [51] and expresses the biological function of NGF [18].

TrkA, one of the NGF receptors (also called neurotrophic tyrosine kinase receptor 1, NTRK1), is a member of the RTKs and is involved in the NGF pathway. Like 22 M. Hiroshima and Y. Sako EGFR, TrkA molecules in homodimers phosphorylate each other on their tyrosine residues upon association with NGF, and activate intracellular signaling molecules. The TrkA dimers are then endocytosed, trafficked to signaling endosomes [21, 32], and transported retrogradely to the soma [37]. Previous studies have demonstrated NGF properties in terms of its mobility, clustering [50], interaction kinetics with its receptor [43], internalization, and transport [8, 30, 49].

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Cell Signaling Reactions: Single-Molecular Kinetic Analysis by Michio Hiroshima, Yasushi Sako (auth.), Yasushi Sako, Masahiro Ueda (eds.)


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