By Sidney Fleischer, Becca Fleischer (Eds.)

ISBN-10: 0121820742

ISBN-13: 9780121820749

The seriously acclaimed laboratory normal, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. in view that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - really a necessary ebook for researchers in all fields of lifestyles sciences

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FIG. 9. The slope of the pipet suction pressure versus projection length of the cell shown in Fig. 8 is used to derive the membrane extensional rigidity. The pressure "loading" and "unloading" phases are reversible, which demonstrates the elastic memory of the cell membrane. This experiment is only valid over the range of aspiration lengths where the membrane surface remains smooth and does not fold or buckle. P, Pipet suction pressure; Rp, pipet inner radius; L, aspirated length inside pipet. static length.

The coefficient of proportionality is the elastic modulus of area dilation (area rigidity) of the red cell membrane which is in the range of 300 to 600 dyn/cm. ~9 This large resistance to area dilation is provided by the superficial bilayer of amphiphilic molecules (lipids, proteins, cholesterol). As will be shown in the next section, aspiration of flaccid red cells requires suction pressures that are 103 times smaller, which shows that the area remains essentially constant for flaccid cell deformation; the low pressures represent membrane extensional and bending rigidities.

Because of the rapid entry into a microvessel, the dynamic rigidity can be at least an order of magnitude greater than the static rigidity of the cell. It is important to note that viscous dissipation in red cell extension is two orders of magnitude greater than dissipation in a lipid bilayer membrane (above the 24 RED BLOOD CELLS [1] acyl chain crystallization temperature). Hence, it is the cytoskeletal meshwork that offers the primary viscous (dynamic) resistance to extension of the cell. Red Cell Membrane Bending (Folding) without Area Dilation or Extension In the previous discussion of membrane extensional rigidity, it was emphasized that planar deformations of the cell surface only occur if the surface contour remains smooth without buckling or folding.

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Biomembranes Part T by Sidney Fleischer, Becca Fleischer (Eds.)

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