By Romano Piras

ISBN-10: 0125572506

ISBN-13: 9780125572507

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W. Ό. Mc. Elrov, A Symposium on Phosphorus Metabolism, The Johns Hopkins Press (1951). 11. R. E. Strange and F. A. Dark, Nature, 177, 186 (1956). 12. H. M. Kalckar, in Polysaccharides in Biology, (Georg. F. ), Universitv of Pennsylvania, Philadelfia, p. 165 (1957). 13. R. E. Trucco, Arch. Biochem. , 34, 482 (1951). 14. A. Munch-Petersen, H. M. Kalckar, E. Cutulo and E. B. Smith, Nature, 172, 1036 (1953). 15. J. L. Strominger, H. M. Kalckar, J. Axelrod and F. Maxwell, J. Amer. Chem. Soc. , 76_, 6411 (1Π54).

T. Mills and E. M. Harper, Biochim. Biophys. Acta, 23^ 662 (1957). 15 LUIS F. LELOIR 30. H. G. Pontis, J. Biol. , 216, 195 (1955). 31. S. Kornfeld and L. Glaser, J. Biol. , 236, 1791 (1961). 32. J. H. Pazur and E. W. Shuey, J. Biol. , 236, 1980 (1961). 33. E. F. Recondo and L. F. Leloir, Biochem. Biophys. Res. Commun. , 6^, 85 (1961). 34. D. G. Comb, F. Shimizu and S. Roseman, J. Amer. Chem. S o c , 80, 653 (1958). 35. E. Cabib, Ann. Rev. , 32, 321 (1963). 36. L. Glaser and D. H. Brown, Proc. Nat.

Glc-X E-Glc-X Glc-Y X E-Glc E-GlcY Incubation of the enzyme with substrate in the absence of a glucosyl acceptor should lead to the accumulation of a glucose-enzyme complex. However, there is an hydrolytic reaction and the complex in its functional form has not been isolated. Silverstein et al. were first able to isolate a denatured form of the complex by adding the enzyme after incubation with 14C-U-sucrose to hot methanol. The incorporated radioactivity corresponds to one mole of glucose per mole of enzyme of MW 80 000.

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Biochemistry of the Glycosidic Linkage: An Integrated View by Romano Piras


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