By Plamen Demirev, Todd R. Sandrin

ISBN-10: 3319260685

ISBN-13: 9783319260686

ISBN-10: 3319260707

ISBN-13: 9783319260709

In the final area century, advances in mass spectrometry (MS) were on the leading edge of efforts to map complicated organic platforms together with the human metabolome, proteome, and microbiome. All of those advancements have allowed MS to turn into a well-established molecular point expertise for microorganism characterization. MS has tested its massive virtue as a swift, exact, and competitively priced strategy for microorganism identity, in comparison to traditional phenotypic concepts. within the final numerous years, functions of MS for microorganism characterization in examine, scientific microbiology, counter-bioterrorism, foodstuff protection, and environmental tracking were documented in millions of courses. Regulatory our bodies in Europe, the U.S., and in different places have authorized MS-based assays for infectious affliction diagnostics. As of mid-2015, greater than 3300 advertisement MS platforms for microorganism id were deployed all over the world in hospitals and scientific labs.

While prior paintings has coated broader methods in utilizing MS to represent microorganisms on the species point or above, this publication specializes in strain-level and subtyping functions. In twelve person chapters, innovators, leaders and practitioners within the box from all over the world have contributed to a entire assessment of present and next-generation techniques for MS-based microbial characterization on the subspecies and pressure degrees. Chapters contain updated reference lists in addition to web-links to databases, advised software program, and different important tools.

The emergence of recent, antibiotic-resistant lines of human or animal pathogens is of outstanding trouble not just to the medical and clinical groups, yet to most of the people besides. advancements of novel MS-based assays for fast id of lines of antibiotic-resistant microorganisms are reviewed within the booklet to boot. Microbiologists, bioanalytical scientists, infectious disorder experts, scientific laboratory and public well-being practitioners in addition to researchers in universities, hospitals, executive labs, and the pharmaceutical and biotechnology industries will locate this publication to be a well timed and priceless source.

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J. Proteomics. 2013;78:273–80. Calderaro A, Piccolo G, Gorrini C, Montecchini S, Buttrini M, Rossi S, Piergianni M, De CF, Arcangeletti MC, Chezzi C, Medici MC. Leptospira species and serovars identified by MALDITOF mass spectrometry after database implementation. BMC Res Notes. 2014;7:330. Camara JE, Hays FA. Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Bioanal Chem. 2007;389:1633–8.

J Clin Microbiol. 2011;49(8):2868–73. Alvarez MTB, Shah DJ, Thulin CD, Graves SW. Tissue proteomics of the low-molecular weight proteome using an integrated cLC-ESI-QTOFMS approach. Proteomics. 2013;13(9):1400–11. Alvarez-Buylla A, Picazo JJ, Culebras E. Optimized method for Acinetobacter species carbapenemase detection and identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2013 51:1589–92. Anhalt JP, Fenselau C. Identification of bacteria using mass spectrometry.

2010; Alvarez et al. 2013). In another hybrid configuration, Q3 is replaced by a linear quadrupole ion trap mass analyzer, which allows ion accumulation for increased sensitivity and MSn capability (Londry and Hager 2003). Orbitrap Mass Analyzer The orbitrap mass analyzer is an ion trap device that provides high accuracy and resolution mass measurement without the need of a magnetic field, (Hu et al. 2005; Zubarev and Makarov 2013) and thus it is more accessible in terms of lab requirements, and initial and operating costs.

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Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance by Plamen Demirev, Todd R. Sandrin

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